neb 10 beta electrocompetent cells (New England Biolabs)
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New England Biolabs
neb 10 beta electrocompetent cells
Neb 10 Beta Electrocompetent Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 646 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neb+10+beta+electrocompetent+cells/NEB+10/bio_rxiv__64898__2026__03__06__710116-158-7-11
Average 99 stars, based on 646 article reviews
Neb 10 Beta Electrocompetent Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 646 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neb+10+beta+electrocompetent+cells/NEB+10/bio_rxiv__64898__2026__03__06__710116-158-7-11
Average 99 stars, based on 646 article reviews
neb 10 beta electrocompetent cells - by Bioz Stars,
2026-10
99/100 stars
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Bioassay:Article Title: Image-based, pooled phenotyping reveals multidimensional, disease-specific variant effects Article Snippet: Paraformaldehyde 20% Solution (Electron Microscopy Sciences, 15713-S) Triton-X 100 (Sigma, Y8787-50ML) Tween-20 (Sigma, P1279-500ML) Glutaraldehyde 25% Solution (Electron Microscopy Sciences, 16210) Deoxynucleotide (dNTP) Solution Mix 10mM (New England Biolabs, N0447L) Ultrapure BSA (Thermo Scientific, AM2616) Ribolock RNase Inhibitor (Thermo Scientific, EO0381) Revertaid H Minus Reverse Transcriptase (Thermo Scientific, EP0451) 5X Reaction Buffer for RT (Thermo Scientific, EP0451) Ampligase Buffer (Biosearch Technologies, SS000015-D3) Taq-IT polymerase (Qiagen, P7620L) Ampligase (Biosearch Technologies, E0001-100D2) Rnase H (New England Biolabs, M0297L) Phi29 DNA Polymerase (Thermo Scientific, EP0094) Glycerol (Fisher Scientific, G33-1) Formamide (Fisher Scientific, BP227-500) Nuclease-Free Water (Thomas Scientific, C001X09) 20X SSC (Invitrogen, 15557036) 10X HBSS (Gibco, 14065-056) Bovine Serum Albumin (Sigma, A2153-100G) Sodium Azide (Sigma, S-8032) Ascorbic Acid (Sigma, A92902-100G) Illumina MiSeq Incorporation Buffer (Illumina, MS-103-1003) Illumina MiSeq v2 Nano 500 kit (Illumina, MS-103-1003) Glass 6-well Plate (Cellvis, P06-1.5H-N) 4’,6-diamidino-2-phenylindole (DAPI; Fisher Scientific, D1306) Sodium borohydride (Sigma, 452882) Phalloidin-CF568 conjugate (Biotium 00044-T) Wheat Germ Agglutinin (WGA)-CF555 conjugate (Biotium 29076-1) Concanavalin A-Alexa Fluor 750 conjugate (Thermo Scientific, C56127) Anti-MAP2 Mouse mAb conjugated to Alexa Fluor 594 (BioLegend, 801802) Anti-phospho-AKT (Thr308) Rabbit mAb (CellSignal, 13038T) Goat Anti-Rabbit IgG H&L cross-adsorbed secondary-Alexa Fluor 647 conjugate (Thermo Scientific, A-21244) .. Corning Untreated 245mm Square BioAssay Dishes (Fisher Scientific, 07-200-600) Carbenicillin Disodium, USP Grade (GoldBio, C-103-250) Kanamycin (Sigma, K1537-25G) Cloning:Article Title: Promoter mutagenesis and a massively parallel reporter screen of the MAPT locus identifies cis-regulatory elements and genetic variation effects Article Snippet: .. Following cloning and electroporation into Article Title: Promoter mutagenesis and a massively parallel reporter screen of the MAPT locus identifies cis-regulatory elements and genetic variation effects Article Snippet: .. Following cloning into pLS-SceI and electroporation into Electroporation:Article Title: Promoter mutagenesis and a massively parallel reporter screen of the MAPT locus identifies cis-regulatory elements and genetic variation effects Article Snippet: .. Following cloning and electroporation into Article Title: Promoter mutagenesis and a massively parallel reporter screen of the MAPT locus identifies cis-regulatory elements and genetic variation effects Article Snippet: .. Following cloning into pLS-SceI and electroporation into Plasmid Preparation:Article Title: Editing stem cell genomes at scale to measure variant effects in diverse cell and genetic contexts Article Snippet: .. Assemblies were electroporated into Article Title: Iterative deep learning design of human enhancers exploits condensed sequence grammar to achieve cell-type specificity. Article Snippet: We amplified the oligo library with primers CY01 and CY02 (Table S6) to add Gibson overhangs; digested and gel purified the plasmid backbone (pMPRA1, Addgene #49349) with SfiI (NEB R0123) to remove its promoter and reporter sequence; then inserted the library cassette into the digested pMPRA1 backbone using the NEBuilder HiFi DNA Assembly kit (NEB E2621). .. This intermediate plasmid was purified with Kapa Pure Beads (Roche KK8002), transformed into Purification:Article Title: Iterative deep learning design of human enhancers exploits condensed sequence grammar to achieve cell-type specificity. Article Snippet: We amplified the oligo library with primers CY01 and CY02 (Table S6) to add Gibson overhangs; digested and gel purified the plasmid backbone (pMPRA1, Addgene #49349) with SfiI (NEB R0123) to remove its promoter and reporter sequence; then inserted the library cassette into the digested pMPRA1 backbone using the NEBuilder HiFi DNA Assembly kit (NEB E2621). .. This intermediate plasmid was purified with Kapa Pure Beads (Roche KK8002), transformed into Transformation Assay:Article Title: Iterative deep learning design of human enhancers exploits condensed sequence grammar to achieve cell-type specificity. Article Snippet: We amplified the oligo library with primers CY01 and CY02 (Table S6) to add Gibson overhangs; digested and gel purified the plasmid backbone (pMPRA1, Addgene #49349) with SfiI (NEB R0123) to remove its promoter and reporter sequence; then inserted the library cassette into the digested pMPRA1 backbone using the NEBuilder HiFi DNA Assembly kit (NEB E2621). .. This intermediate plasmid was purified with Kapa Pure Beads (Roche KK8002), transformed into Clone Assay:Article Title: Interactions Between Dietary Metabolites and Regulatory Risk Variants for Human Colon Cancer Article Snippet: The first round of PCR added the minimal promoter, followed by a second round of PCR to add 15-bp random barcodes downstream of the promoter. pLS-SceI (Addgene, 137725) was digested with AgeI and SbfI and amplified library inserted into the digested vector using NEBuilder HiFi (NEB, E2621). .. Cloned library was electroporated into |
